The Determination of T-Lymphocyte Percentage in Blood from Angle Resolved Light Scattering

Read more This work shows the possibility of determination of T-lymphocyte percentage in blood by light scattering pattern only. The analysis of lymphocytes was carried out by scanning flow cytometry with measurement light scattering and specific fluorescence simultaneously. The fluorescence was caused from fluorochrom radiation which was connected with monoclonal antibody. The analysis of light scattering from different lymphocyte subpopulations allowed reveal the dependence distribution asymmetry of T-lymphocyte percentage. This method resulted in determination of T-lymphocyte percentage with mistake less then 4,5 %. The analysis by this method takes 10–15 minutes as the same time immunofluorescence analysis takes several tens of minutes. Thus, our method develops a potential of scanning flow cytometry in carrying out bulk hematological analysis.

Keywords:
flow cytometry, inverse light scattering problem, light scattering indicatrix, T-lymphocytes.
УДК 535.36

The Determination of T-Lymphocyte Percentage in Blood from Angle Resolved Light Scattering