APPLICATION OF THE SOLUTION OF THE INVERSE LIGHT SCATTERING PROBLEM FOR CHARACTERIZATION OF MONONUCLEAR CELLS

Read more In this manuscript we propose two methods to solve inverse light scattering problem for single particles, which can be described as a coated sphere. The efficiency of the methods is illustrated by characterization of lymphocytes and stem cells using light scattering patterns obtained with scanning flow cytometer. Both methods, spectral and global optimization, were used to obtain diameters and refractive indices of the cytoplasm and the nucleus of mice embryo stem cells and human lymphocytes. These results agree with data obtained from other studies. Determination of these parameters is important for diagnostics of pathological states of lymphocytes and differentiation status of embryo stem cells. Moreover, methods described in this manuscript are applicable to all mononuclear cells. We also considered limitations of these methods and their possible improvements.

Keywords:
flow cytometry, inverse light-scattering problem, light scattering pattern, lymphocytes, lymphocyte morphology, global optimization, stem cells, spectral method.
УДК 535.36

APPLICATION OF THE SOLUTION OF THE INVERSE LIGHT SCATTERING PROBLEM FOR CHARACTERIZATION OF MONONUCLEAR CELLS