6. Chemical, Biological and Medical Physics

DETERMINATION OF DYNAMICAL CHARACTERISTICS OF THROMBOCYTES THROUGH INITIAL STAGE OF THEIR AGGREGATION

Irina V. Kolesnikova
1. JSC «Vektor-Best» Novosibirsk, Russian Federation
Vyacheslav M. Nekrasov
1. Institute of Chemical Kinetics and Combustion, SB RAS Novosibirsk, Russian Federation
2. Novosibirsk State University Novosibirsk, Russian Federation
Tatyana N. Sherstova
1. Institute of Chemical Kinetics and Combustion, SB RAS Novosibirsk, Russian Federation
2. Novosibirsk State University Novosibirsk, Russian Federation
The material was received by the Editorial Board: 05.03.2009
In the work, a method of application of scanning flow cytometry for investigation of the aggregation dynamics of invitro activated thrombocytes is presented. As a result, the rate constant of creation of initial aggregates, – dimers, – from single thrombocytes, and the effective steric factor of aggregation, which characterized the thrombocytes activation degree, are determined. The study reveals diagnostic potential of the scanning flow cytometer in determination of relative concentrations of aggregates and non-aggregated thrombocytes in a sample by measuring light-scattering signals from single particles and evolution of the distribution function of the particles on the signals. Theoretical treatment of the experimental data is based on modeling the particles aggregation kinetics with the use of Smoluchovski equation.

Keywords:
thrombocytes, aggregation kinetics, light scattering, scanning flow cytometer.
УДК 577.31

DETERMINATION OF DYNAMICAL CHARACTERISTICS OF THROMBOCYTES THROUGH INITIAL STAGE OF THEIR AGGREGATION
References: Kolesnikova I.V., Nekrasov V.M., Sherstova T.N., Tsvetovskaya G.A., Chikova E.D., Maltsev V.P., Chernyshev A.V. DETERMINATION OF DYNAMICAL CHARACTERISTICS OF THROMBOCYTES THROUGH INITIAL STAGE OF THEIR AGGREGATION. Vestnik NSU. Series: Physics. 2009, vol. 4, no. 2. P. 69–77 (in Russ.). DOI: 10.54362/1818-7919-2009-4-2-69-77