INFLUENCE OF OLIGONUCLEOTIDE-STOPPER ON SIZE AND THERMAL STABILITY OF CONCATEMER DNA COMPEXES

Multicomponent polymeric associates based on nucleic acids are well used in drug and gene delivery into cells, in DNA fragments manipulations, in amplifying of the molecular hybridization signal, etc. Nevertheless, the formation of these systems is not well studied. In this paper, influence of a component that terminates concatemer elongation on a length and thermal stability of the multicomponent complex was studied. We have shown that addition of a oligonucleotide-stopper to the polymeric complex consisting of two oligonucleotide species decreases the length of concatemer complexes proportionally to the stopper concentration. Effect of the stopper on melting temperature was shown to be concentration dependent. We have proposed a thermodynamic model, which allows one to predict the thermal stability and the length distribution of the concatemeric complex consisting of two types of oligonucleotides in the presence of the stopper. We have also determined the length of polymeric complexes, which makes the maximum contribution to the intensity of the analyzed signal.

Keywords:
concatemer, DNA, oligonucleotide, stopper, thermodynamics, self-association.
УДК [536.7:544-971.62:577.323]:577.113.4/.7

INFLUENCE OF OLIGONUCLEOTIDE-STOPPER ON SIZE AND THERMAL STABILITY OF CONCATEMER DNA COMPEXES