EFFECT OF TERAHERTZ LASER IRRADIATION ON ERYTHROCYTE AGGREGATION IN HEALTHY BLOOD

The study is devoted to a topical field of research – the interaction of terahertz radiation with biological structures. This paper examines the impact of the Novosibirsk terahertz free electron laser on the aggregation and morphology of red blood cells of prepared blood samples in vitro. The aim of this study was to determine the safe action of the laser radiation on red blood cells and the identification of specific effects that arise in the erythrocytes under the action of terahertz radiation and lead to non-destructive disaggregation of individual cells of erythrocytes. The methodology of sample preparation of the objects, obtaining and analyzing micrographs based on the classification of red blood cells and particles model colloidal system of the area into classes corresponding to the individual and aggregate particles, was developed. Found that 5sec exposure of 200 mkl sample of red blood cells by the terahertz radiation with wavelength of 130–146 mm and an average power density of 8–10 W/cm² does not lead to visible changes in cell morphology and the number of aggregated particles. The exposure of more than 25 seconds can lead to lysis of erythrocytes. When exposed for 10–15 sec the average number of erythrocytes in the aggregates is reduced by 10–15 % in some cases – up to 80 %.

Keywords:
terahertz radiation, erythrocyte aggregation, free electron laser.
УДК 537.531.2:016; 616.155.1-073.213(083.131)

EFFECT OF TERAHERTZ LASER IRRADIATION ON ERYTHROCYTE AGGREGATION IN HEALTHY BLOOD